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Core Course Descriptions
Bioprocess Engineering I: Kinetics, Energetics and Bioreactor Design
(MBIOTECH 476-1, 1 Unit)
Enzymes and enzyme mechanisms; Kinetics of free and immobilized enzymes; Stoichiometry of major metabolic pathways and overall cellular stoichiometry; Cellular energetics and thermodynamics; Microbial kinetics; Bioreactor operations - batch, continuous, and fed-batch modes; Models for cellular systems - unstructured, structured, lumped and population models; Mixing and mass transfer in bioreactors; Heat transfer and sterilization; and Applications and examples - fermentation, cell culture, biotransformations.
Bioprocess Engineering II: Properties and Separation of Biologically Produced Molecules
(MBIOTECH 476-2, 1 Unit)
Properties and stability of biological products; Cell lysis and flocculation; Centrifugation; Membrane processes for concentration and separation of biological products; Precipitation and crystallization of bioproducts; Extraction (organic and aqueous two phase); Chromatography methods for separation of biologicals; Bioseparation process design, analysis, and simulation; Analytical methods in bioprocess development.
Biotechnology Laboratory
(MBIOTECH 401, 1 Unit)
This course is designed specifically for the students of the MBP to provide them with practical experience in basic biotechnology. It will cover the following topics: Plasmid DNA isolation and restriction analysis; DNA and protein electrophoresis; Molecular cloning; Quantification of DNA and protein; Amplification and analysis of DNA by polymerase chain reaction; Separation of proteins and DNA by gel electrophoresis; Western blot hybridization; Mammalian cell culture techniques.
Bioprocess Engineering Laboratory
(MBIOTECH 402, 1 unit)
This will provide in practicum training in bioreactors and downstream unit operations in biotechnology. It will include the following experiments: Bacterial and mammalian cell culture in bioreactors; cross-flow filtration, ultrafiltration, centrifugation, affinity chromatography, ion-exchange chromatography and, aqueous two-phase extraction.
Balances and Kinetics for Bioprocessing
(MBIOTECH 301, 1 Unit)
The first in a sequence of two transitional courses is designed to introduce non-engineers to the fundamentals of mass and energy balances, and kinetics. An important objective of this course is to prepare the students for the core sequence of two advanced Bioprocess Engineering courses (MBIOTECH 476-1, -2). MBIOTECH 301 will cover the following topics: Chemical/biochemical Processes and process variables; Mass balances; Energy balances; Mole balances; Conversion and reaction sizing; Rate laws and stoichiometry; Reactor design; and collection and analysis of data.
Fluids and Mass Transfer for Bioprocessing
(MBIOTECH 302, 1 Unit)
The second in a sequence of two transitional core courses is designed to introduce non-engineers to the fundamentals of fluids and mass transfer. An important objective of this course is to prepare students for the core sequence of two advanced Bioprocess Engineering courses (MBIOTECH 476-1, -2). MBIOTECH 302 will cover the following topics: Basic laws of physics, units, dimensions, dimensionless analysis; Fluid statics and applications; Classification of fluid flow, shear rate, shear stress, viscosity, turbulence; Mass and energy balance in fluid flow; mechanical-energy balance and the Bernoulli equation; Flows in pipes; Flow through porous beds; Motion of particles in fluids; Mixing and agitation; Fundamentals of mass transport between phases; Diffusive and convective transport; equimolar and unicomponent diffusion; Mass transfer in fluid flow; Equilibrium staged operations; Gas-liquid mass transfer; and Applications to biological processes.
Microbial Biotechnology
(MBIOTECH 421, .5 units)
Microbial Biotechnology will explore the application and use of microorganisms to the benefit of human society. Microbiological fundamentals of prokaryotic, eukaryotic, and viral microorganisms will be discussed, including isolation, preservation, safety, strain improvement, and genetic engineering. The contributions of microorganisms to the production of foods, antibiotics and biopharmaceuticals, industrial and research enzymes, chemical feedstocks, and biofuels will be communicated. Environmental applications of microorganisms will be reviewed- wastewater treatment, organic slurry digestion, treatment of solid and gas wastes, and soil remediation.
Biotechnology Applications of Immunology
(MBIOTECH 422, .5 units)
Biotechnology Applications of Immunology will review the basics of the immune system- cell types and humoral vs. cellular responses- and will reveal how manipulation of the immune system and its components is used by the biotechnology industry to treat human disease. Topics discussed include B and T cells, immune effector cells, stem cells and therapy, cell sorting, vaccines, antibodies, therapeutic proteins, autoimmune and transplant rejection therapy, cancer biology and treatment, and gene therapy.
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